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Required DOT Drug Tests & Cutoffs <br />TYPE OF DRUG <br />INITIAL TEST <br />CONFIRMATORY TEST <br />CONFIRMATORY TEST <br />Initial Test Analyte <br />Cutoff Concentration" <br />Analyte <br />Cutoff Concentration <br />Marijuana metabolites MCA 10 <br />50 n ImL11 <br />THCA <br />15 ng/mL <br />Cocaine metabolite <br />150 ng/mLl I <br />Benzoylecgonine <br />100 ng/mL <br />Benzo lec onine <br />Opioids: <br />Codeine/Morphine <br />2000 ng/mL <br />Codeine <br />2000 ng/mL <br />Morphine <br />2000 ng/mL <br />Hydrocodone/Hydromorphone <br />300 ng/mL <br />Hydrocodone <br />100 ng/mL <br />Hydromorphone <br />100 ng/mL <br />Oxycodone/Oxymorphone <br />100 nglmL <br />Oxycodone <br />100 ng/mL <br />Oxymorphone <br />100 ng/mL <br />6-Acet Imor hine <br />10 n ImL <br />B-Acetylmolrphine <br />10 n ImL <br />Phencyclidine <br />25 n /mL <br />Phencyclidine <br />25 n ImL <br />Amphetamine/Methamphetamine <br />500 nglmL <br />Amphetamine <br />250 ng/mL <br />Methamphetamine <br />250 ng1mL <br />MDMA 12IMDA1' <br />500 ng/mL <br />MDMA <br />250 ng/mL <br />MDA 1 <br />250 n /mL <br />Table 1 <br />For grouped analytes (i.e., hvo or more analytes that are in the same drug class and have the same initial test cutoff): <br />hnnrunoassav: The test must be calibrated with one anayete from the group identified as the target analyte. The cross -reactivity <br />of the immunoassay to the other analyte(s) within the group must be 80 percent or greater. If not, separate immunoassays must be <br />used for the analytes within the group. 4 <br />Alternate teelinologv: Either one analyte or all analytes from the group must be used for calibration, depending on the <br />technology. At least one analyte within the group must have a concentration equal to or greater than the initial test cutoff or, <br />alternatively, the sum of the analytes present (i.e., equal to or greater than the laboratory's validated limit of quantification) must <br />be equal to or greater than the initial test cutoff. <br />10 An immunoassay must be calibrated with the target analyte Delta-9-tetrahydrocannabinol-9-carboxylic acid (THCA) <br />1 ` .-Alternate technology (THCA and ben:ovlecgonine): When using alternate technology to test for THCA and Benzoylecgonine, <br />the screening and confirmatory test cutoff concentrations must be the same respectively (i.e., 15 ng'mL for THCA and 100 nQ/mL <br />for Benzoylecgnine). <br />12 Methylenedioxymethantphetamine (MDMA) <br />13 Methylenedioxyamphetamine (MDA) <br />Premium Concrete Services, Inc.- PHMSA âť‘RUGIALCOHOL PLAN 25 <br />NATIONAL MAPLIANCE MANAGEMENT SERVICE, INC (NCAIS) 2G 11 lupdate 2C2l} The NGNIS plan is <br />+P ibhlrrl of n rn lerr,4 rnnvr oht and m nrntoeterl by nnnvevht lawc in Ihn I 9 ahA nlcn,ihrrn All rinhl% <br />