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2. Fish community sampling will be conducted using two different sampling approaches: <br />Index sampling (to be used for long-term trend analysis) and Investigative sampling (to investigate <br />areas that are not sampled frequently). Index sampling shall be conducted twice annually. <br />Investigative sampling shall be conducted once annually. Habitat surveys will also be conducted at <br />all sampling locations. <br />3. Fish tissue samples will be collected during surveys and analyzed for the following: <br />polychlorinated biphenyls (PCB's); mercury (Hg); organochlorine pesticides; silver (Ag); cadmium <br />(Cd); copper (Cu); nickel (Ni); lead (Pb); and zinc (Zn). <br />4. Sampling sites, and the type of sampling, shall be conducted on an annual (calendar <br />year) basis as described below: <br />a. Four or five (4-5) boat sites on the St. Joseph River (three index samples and <br />one or two investigative samples). Fish tissue will be collected from a minimum of two (2) sites. <br />b. Two or three (2-3) tote barge sites on Juday Creek (one or two index samples <br />and one investigative sample). <br />C. One or two (1-2) tote barge/backpack sites on Bowman Creek (one index <br />sample and one investigative sample). <br />d. A minimum of two (2) investigative sites on other area streams/water bodies <br />as needed and as time allows. <br />e. Macroinvertebrate communities will be sampled at a minimum of two (2) <br />locations on the St. Joseph River, one or two (1-2) locations on Juday Creek and one to two (1-2) <br />locations on Bowman Creek. <br />5. From each site, one specimen from each species of fish collected shall be retained or <br />—2— <br />